Dr. Patrick Pirrotte

Confocal/Apotome microscopy, transmission electron microscopy, ion microscopy and respective sample preparation techniques (cryofixation AFS, CPC, CFD), ultramicrotomy, immunocytochemistry.

Mass spectrometry: Maldi-TOF and dynamic SIMS (NanoSIMS).

Development and use of new isotopic markers for SIMS and Maldi-TOF.

Cell culture, bacteria culture, proteomics (2D DIGE gels, western blots, protein extractions and separation).

Software development for the processing and analysis of imaging and sequencing data.

Information Management Centre / Integrated Biobank of Luxembourg (Project Manager)

Luxbg. Proteomics Society

LuciLinx: Luxbg. Bioinformatics Society

Identification of seven new post translational modifications in Measle virus nucleocapsid protein. Prodhomme E, Fack F, Revets D, Pirrotte P, Kremer J, Muller CP. Molecular & Cellular Proteomics, to be submitted.

Characterisation of a new DNA binding domain deficient Chicken Ovalbumin Upstream Promoter-Trasncription Factor IIΔ isoform in the human brain. Schote BA, Bechet T, Pirrotte P, Turner JD, Muller CP. PLOS One. Submitted July 2009

Detection of differentially modified pathogen proteins by Western Blot after 2-D gel electrophoresis and identification by Maldi-Tof-Tof. Fack F, Kessler J, Pirrotte P, Kremer J, Revets D, Ammerlaan W, Muller CP. COST B28, 2008.

Biological Applications of the NanoSIMS. Pirrotte P. Université Louis Pasteur, Doctoral Thesis, November 2007.

Secondary Ion Mass Spectrometry in Life Sciences. Pirrotte P, Guerquin-Kern JL, Audinot JN, Migeon HN and Muller CP. Book chapter in Emerging Imaging Techniques. Eds.: M Faupel, P Smigielski, R Grzymala. FontisMedia ISBN 2-88476-008-3.

Image Gallery Database: Multi-User Image Management Software. Pirrotte P and Mutterer J. Microscopy and Analysis, 2004, 18(1).

3rd prize Image processing using ImageJ Video Tutorial Competition. Second ImageJ User & Developer Conference, 6-7.11.2008.

New tools for metadata extraction and image analysis for SIMS and confocal microscopy: applications in biology using isotopically labelled probes. Pirrotte P, Mutterer J, Audinot JN, Muller CP , Annual symposium of the Luxembourg Bioinformatics Network (LuciLinX), 16.10.2008.

Stable isotope labelling of antigens for the study of phagocytic processes by NanoSIMS. Pirrotte P, Fack F, Revets D, Audinot JN, Migeon HN, Muller CP, Imvie3, Paris, France, 20-21.06.2006.

ImageJ plugin based on ImageIO to extract images and data from Cameca Secondary Ion Mass Spectrometer data files. Pirrotte P, Audinot JN, Muller CP. ImageJ User and Developer Conference, CRP-HT, Luxembourg, 18-19.5.2006. Analyse de tissues biologique par Nano/SIMS.

Pirrotte P and Muller CP. BIOSIMS Luxembourg Meeting, 11.05.2005.

Biological Imaging with NanoSIMS. Pirrotte P, Audinot JN, Migeon HN, Lasbennes F, Muller CP. Congrès d’Imagerie Imvie2, Strasbourg, France, 01-03.03.2005.